Temperature-responsive polymer (PIPAAm)

Does the temperature-responsive polymer (PIPAAm) come off with cells?
The polymer is covalently bonded to the surface of the polystyrene therefore it does not come off as long as the product is used in the "normal course of cell culture".
Does this polymer make cells easier to attach when coated?
This polymer allows detachment of cells proteins without causing any damage to them by mere reduction of temperature. Hence it does not make the surface of the dish easier to attach for cells.
Why cells are easier to detach when the temperature is reduced?
The polymer immobilized surface changes from slightly hydrophobic to super hydrophilic state across the transition temperature of 32℃ (LCST=lower critical solution temperature). The cells lose their anchorage when the surface becomes hydrophilic, prompting cells to detach.
Any constraints as to the materials to be coated with this polymer?
At the moment, the polymer can only be fixed to the organic substance such as platics without incurring any additional research/development. However we can not fix polymer to the paper though is is also organic substance. Apart from this, we would require additional research for fixing polymer to the glass or steels.
Any constraints as to the shape of the surface to be coated with this polymer?
If the organic substance to be coated with polymer is ball-shaped or round shaped, we need some time to develop suitable technique.
Can you change the LCST (lower critical solution temperature) of 32℃?
Yes, it is possible to change LCST. For further information please contact here.
Is there any cell toxicity of the PIPAAm?
The safety of the polymer has been subjected against the rigorous test in accordance with ISO 10993.