Application Data

Application data

  1. Detachment of cells by control of temperature.
  2. Comparison of re-attachment kinetics for harvested A-549 cells between UpCell®/RepCell® (UpCell® with Grid) and trypsin.
  3. Comparison of harvested extra cellular matrix between UpCell®/RepCell® (UpCell® with Grid) and dispase (E-cadherin).
  4. Comparison of flow cytometry application between UpCell®/RepCell® (UpCell® with Grid) and trypsin.
  5. Comparison of detachment of macrophage between UpCell®/RepCell® (UpCell® with Grid) and trypsin.
  6. Comparison of re-attachment of macrophage between UpCell®/RepCell® (UpCell® with Grid) and trypsin.
  7. Protocol for macrophage collection in single cell suspension
  8. Protocol for dendritic cell collection in single cell suspension.
  9. Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell® 3.5cm.
  10. Application data for mono-layered cell-sheet.
  11. Application data for multiple layered cell-sheet (3D tissue constructs).

Recommended operating procedure

  1. Standard Operating Protocol (SOP) for collection of cell-sheet.
  2. Key issues to be aware of when collecting cell-sheet.
  3. Protocol to make frozen section or paraffin-embedded section with cell-sheet.
  4. The protocol for collagen coating on the surface of UpCell® (For the case of HUVEC cells).
  5. Soft-agar assay of using methylcellulose for HydroCellTM.

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